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KMID : 1147720120050060319
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Journal of Acupuncture and Meridian Studies
2012 Volume.5 No. 6 p.319 ~ p.322
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Inhibitory Effects of Isoflavonoids on Rat Prostate Testosterone 5α-Reductase
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Bae Mi-Jeong
Woo Mi-Jin
Kusuma Irawan Wijaya
Arung Enos Tangke
Yang Chae-Ha
Kim Yong-Ung
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Abstract
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Testosterone 5α-reductase inhibitors represent important therapeutic drugs for use against androgen-dependent diseases such as benign prostatic hyperplasia, male pattern baldness, and acne. We have searched for inhibitors of rat prostate testosterone 5α-reductase in the cultured broths of many kinds of soil bacteria, and have found that cultured soybean-casein digest broths of certain bacterial strains have a potent inhibitory effect on the enzyme. We tested 10 selected isoflavonoids, including isoflavones and O-methylated isoflavones, for inhibitory effects on rat prostate testosterone 5α-reductase to determine the important structural elements for inhibition of the enzyme. Genistein, biochanin A, equol, and 3′,4′,7-trihydroxyisoflavone showed considerably higher inhibitory effects whereas daidzein, formononetin, glycitein, prunetin, ipriflavone, and 4′,7-dimethoxyisoflavone showed lower inhibitory effects. The IC50 values of genistein, biochanin A, equol, 3′,4′,7-trihydroxyisoflavone, and riboflavin, a positive control, for rat prostate testosterone 5α-reductase were 710 μm, 140 μm, 370 μm, 690 μm, and 17 μm, respectively. Daidzein, genistein, biochanin A, formononetin, and equol are already known to be testosterone 5α-reductase inhibitors, but this is the first characterization of 3′,4′,7-trihydroxyisoflavone as an inhibitor of the enzyme.
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KEYWORD
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bacteria, isoflavone, O-methylated isoflavone, soybean, testosterone 5α-reductase inhibitor, 3′,4′,7-trihydroxyisoflavone
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